By Rosella Campbell


With the continuous development and improvement of most technologies, it has already formed new dimensions. A technology in the medical field has been formulated new aspects. With the existence of diseases and anomalies, ELISA technology has been used in this matter. The Cardiac ELISA kits are one of the most useful and a good one. These are tools good in working with the hearts of most animals and to detect defects on it.

The procedure in identifying illnesses depends on a certain enzyme reaction that provides better results by giving a particular color changes. The antibodies and the enzymes are mixed with antigens and eventually change its color in indicating an existence of the reactions. Through this test, most researchers can detect both antibodies and antigens.

Certain exercises are able to identify certain levels of antibodies and antigens in most patients. These are useful in determining a particular disease before developing chronic problems. Doctors are able to work early stages of a development. This because of the experiments conducted using the equipment. Thus, most patients are also able to eliminate the problems in a great value.

Working on the tools properly can give a sensible reaction that also provides better result that is capable of making a detailed reading at a given period of time. If a given tool contains sensitivity manners, it can also give changes that may be a result of certain reactions of the reagents and samples. It contains efficiency and accuracy that provides assurance in obtaining a free error or mistake condition. Most of them are working to resolve a certain problem.

It is important for every equipment to have a stability aspect. Thus, to obtain a certain stability level, one must lessen the rate of loss in this activity. This may be possible using proper storage. This stability can be attained by reducing a specific effect to its setup.

This means that the temperature, pressure and humidity need to agree the standard lab requirements. There must be someone who controls the incubator temperature. If one person is only allowed to work with the research from the start up to the end, it can easily attain stability.

In conducting an experiment, you only need to prepare reagents, standards and samples. The researcher should add a small quantity of samples and incubate for an hour. After this, he or she must then pursue the mixture and wash it for three times. Next is to add a solution and incubate again for twenty to twenty five minutes. The final step is to add a stop solution and do some readings.

The principle applied here is the enzyme sandwich. The plates within the testing tools are well coated with an antibody for the heart defects. The samples are then placed on the plates in a proper manner. The main content here is that most samples are particularly a biotin conjugate antibodies. Before the incubation, conjugate of Avidins are added into the plates.

Once an added substrate solution is applied, the wells will be filled with type 3 of tropin I. The reagents then will change its colors. Mos of these reactions are brought specifically at the end with an additional solution of sulfuric acid. Its color is always changing and measured in a specific style.




About the Author:



0 comments :