Clodronate Liposome For Suppressing Macrophage Activity

Clodronate Liposome is used in different research laboratories. Clodronate is used as a treatment for different diseases, especially for cancer metastases. It effects further development of metastases, reduces pain and decreases the risk of fractures. Since this drug cannot be delivered to tissues without suppressing macrophage activity, this nano technology is used for this purpose.

Liposomes are lipid-based nanoparticles. Liposomal encapsulated drugs are mostly used in academic research laboratories. Some new anti-cancer compounds such as this one require a liposome delivery system to be closed in vivo. Target organs for this particular compound are kidneys and bones.

Clodronate liposome suspension should be injected to targeted tissues. While manipulating this type of suspension, some measures of precaution are necessary, such as chemical resistant gloves and clothing, safety goggles and respirators. This compound can be harmful if inhaled, absorbed through skin, swallowed or if it get in contact with your eyes. It causes different irritations, especially to mucous membranes, and you should be very careful with it.

If you get in any type of direct contact with it, make sure to follow the procedure. If it get in contact with your eyes, you should flush them with clean water for some time repeatedly, approximately fifteen minutes or so. If it get in touch with your skin, wash it with water and soap. If you inhale it, go outside on fresh air immediately. For more serious exposures, maybe you should have a respirator or even oxygen nearby. If you swallow it, rinse your mouth and call a doctor.

Macrophages are very important, because they regulate immune reactions and some other, non-phagocytic cells function. Their primary function is to ingest and digest unfamiliar macromolecules and other microorganisms. In order to override their function, they use liposomal encapsulated compounds and drugs.

Liposomes encapsulate clodronate, and they are used for delivering it to tissues, in this case kidneys and bones. They will be ingested and digested by macrophages. After that, active compound will be released and accumulated. This will create macrophage depleted tissues or organs, allowing in vivo research of different functional aspects of macrophages.

Different diseases can be researched this way, using clodronate liposome. Some autoimmune diseases, transplantation and neurological disorders can also be researched using this type of macrophage suppression. The same method can actually be used for in vitro depletion of macrophages, but it is primarily designed for in vivo researches.

The suspension should be kept at 4 degrees of Celsius, up to one month. The important thing to remember is that it should never be frozen, or exposed to temperatures over 30 degrees. It is also important to wait until this suspension reach room temperature before injecting it to your animals. Other ways your results could be different than expected.

It is also important to shake the suspension until it becomes homogeneous. You should shave the skin and cleanse it with alcohol, to make sure it is disinfected. You should use sterile syringe for each animal. If you use the same one, you might get uneven dosages. Follow the procedure for injecting clodronate liposome to your test animals.

About the Author:    Adele Madden 

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